This facility provides a broad platform for semi-automated crystallization using different approaches. State-of-the art liquid-handling systems enable fast and easy preparation of crystallization screens and setups. A free interface diffusion system is available for crystallization setups at the nanoliter scale. The results of crystallization experiments can be monitored using an automated crystallization-plate incubation and imaging system. The facility is temperature-stabilized and provides lab space and microscopes for manual setups, manipulation and preparation of protein crystals for synchrotron measurements, as well as different temperature-controlled incubators and storage possibilities. The facility ensures that the Gene Center is highly competitive in the X-ray structure analysis of challenging fragile and scarce macromolecular complexes.