Cryo-Electron Microscopy

This state-of-the-art facility permits large-scale data collection for single particle reconstruction. It is set up to generate de novo 3D structures of protein complexes by using low-dose tomography and random conical approaches. The sample preparation unit is equipped for ultra-thin sectioning of embedded specimens, generation and glow-discharging of thin carbon films and the automated vitrification of samples (Vitrobot Mark IV). A 100-kV Morgagni routine transmission EM can be used for rapid sample screening and analysis of standard biological specimens. The 120-kV Spirit G12 EM with cryo-capabilities enables rapid data collection for medium-resolution analysis. For high-resolution cryo-EM we use the 300-kV Titan Krios EM, currently the most advanced microscope on the market. Custom-fitted automatic acquisition software combined with a 4k x 4k CCD camera allow for high-throughput data collection. Finally, a tabletop 5-kV LVEM5 low-voltage EM is available for initial sample characterization. Taken together, this facility allows us to carry out ultrastructural research at the highest international level.

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